A potential strategy for the treatment of spinal cord injuries represents the cell therapy with olfactory ensheathing- or stem cells as well as with mesenchymal stem cells. Such cell therapies could enable the replacement or support of injured glial cells and neurons. The techniques of cell transplantations are different. However, one important common feature is that all cells will be in contact with cerebrospinal fluid (CSF). For this reason, this project clarifies in advance what effect the CSF may have on the transplanted cells in order to identify possible adverse reactions (such as osteogenic differentiation) in addition to therapeutically desired effects.
Optimal isolation and purification methods for olfactory stem- and ensheathing-cells have been identified. Here, the influence of CSF on the cells, such as on the viability, proliferation and also cell functionality should be analysed.
For the planned project, the olfactory cells should be enriched in complex procedures from nasal mucosal biopsies. These techniques are already established. Mesenchymal stem cells are purchased commercially. Cerebrospinal fluid is provided by the cooperation partner of neurosurgery and neurotraumatology (Prof. Martínez-Olivera). The following methods are used:
-cross sectoral-Type of hazard:
rehabilitationDescription, key words:
stem cells, spinal cord, cell therapy