Functional characterization of particles, including fibers and nanomaterials

Project No. IPA 132

Status:

ongoing

Aims:

In order to model the mode of action of inflammatory particles, the Particle-Induced Cell Migration Assay (PICMA) was developed at the Institute for Prevention and Occupational Medicine (IPA). The test displays acute inflammatory particle effects with high differentiation, sensitivity and reproducibility, and permits classification of particles into those without effect, with medium and with strong effect.

Activities/Methods:

Examination of further particles and comparison with animal and human data will be used to test the transferability of the PICMA results.

Results:

Furthermore, parameters used as measures of inflammatory processes in animal or human studies can be confirmed experimentally by means of PICMA. Early non-toxic effects, indicating particle effects which in the case of chronic exposure may result in serious diseases, can be identified by means of this new method.

Last Update:

28 Feb 2023

Project

Financed by:

Institut für Prävention und Arbeitsmedizin der Deutschen Gesetzlichen Unfallversicherung - Institut der Ruhr-Universität Bochum (IPA)

Research institution(s):

Institut für Prävention und Arbeitsmedizin der Deutschen Gesetzlichen Unfallversicherung - Institut der Ruhr-Universität Bochum (IPA)

Branche(s):

-cross sectoral-

Type of hazard:

work-related health hazards, dangerous substances

Catchwords:

dust, fibers, particles

Description, key words:

occupational health risks, hazardous substances, dust, fibers, particles

Contact

ipa@dguv.de

Further information

Loza, K.; Schremmer, I.; Bünger, J.; Westphal, G.A.; Köller, M.; Epple, M.; Sengstock, C.: Barium sulfate micro- and nanoparticles as bioinert reference material in particle toxicology. Nanotoxicology Nanotoxicology. 2016, 10:1492-1502.
Monsé, C.; Westphal, G.A.;, Raul, M.; Jettkant, B.; van Kampen, V.; Kendzia, B.; Schürmeyer, L.; Seifert, C.E.; Marek, E.M.; Wiegand, F.; Rosenkranz, N.; Wegener, C.; Merget, R.; Brüning, T.; Bünger, J.: No inflammatory effects after acute inhalation of barium sulfate particles in human volunteers. BMC Pulm Med 2022; 22.
Monsé, C.; Raulf, M.; Jettkant, B.; van Kampen, V.; Schürmeyer, L.; Seifert, C.E.; Marek, E.M.; Westphal, G.A.; Rosenkranz, N.; Merget, R.; Brüning, T.; Bünger, J.: Health effects after inhalation of micro- and nano-sized zinc oxide particles in human volunteers. Arch Toxicol 2021; 95: 53-65 DOI 10.1007/s00204-020-02923-y
Olejnik, M.; Breisch, M.; Sokolova, V.; Loza, K.; Prymak, O.; Rosenkranz, N.; Westphal, G.A.; Bünger, J.; Köller, M.; Sengstock, C.; Epple, M.: The effect of short silica fibers (0.3 μm 3.2 μm) on macrophages. Sci Total Environ 2021; 769: 144575 DOI 10.1016/j.scitotenv.2020.144575
Olejnik, M.; Kersting, M.; Rosenkranz, N.; Loza, K.; Breisch, M.; Rostek, A.; Prymak, O.; Schürmeyer, L.; Westphal, G.A.; Köller, M.; Bünger, J.; Epple, M.; Sengstock, C.: Cell-biological effects of zinc oxide spheres and rods from the nano- to the microscale at sub-toxic levels. Cell Biol Toxicol 2021; 37: 573-593 DOI 10.1007/s10565-020-09571-z
Schremmer, I.; Brik, A.; Weber, D.G.; Rosenkranz, N.; Rostek, A.; Loza, K.; Brüning, T.; Johnen, G.; Epple, M.; Bünger, J.; Westphal, G.A.: Kinetics of chemotaxis; cytokine; and chemokine release of NR8383 macrophages after exposure to inflammatory and inert granular insoluble particles. Toxicol Lett 2016; 263: 68-75 DOI: 10.1016/j.toxlet.2016.08.014
Tölle, L.; Monsé, C.; Rosenkranz, N.; Haibel, N.; Walter, D.; Bünger, J.; Hopp, M.; Westphal, G.A.: Characterization of fiber dust resulting from recycling of carbon fiber-reinforced thermoplastics (CFRP) and their cell toxicity. MSCE 2022; 10: 1-16 DOI 10.4236/msce.2022.107001
Westphal, G.A.; Schremmer, I.; Rostek, A.; Loza, K.; Rosenkranz, N.; Brüning, T.; Epple, M.; Bünger, J.: Particle-induced cell migration assay (PICMA): a new in vitro assay for inflammatory particle effects based on permanent cell lines. Toxicol in Vitro 2015; 29: 997-1005 DOI: 10.1016/j.tiv.2015.04.005